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How to make liquid culture

source

source

Needed:

• wide mouth jar
• modified lid
• honey or simple sugar (corn syrup or dextrose)
• pressure cooker
• Stir plater and magnetic stirrer bar
  
• scale
• 70% ISO alcohol
• Cutlure - syringe, liquid cultuire, plate culture or any source of myceleum

Directions

Variety of nutritional source

It can belp to add some other food sources so that the culture has more variety, like:

• rye berries
• potato flakes
• some coir
• nutritional yeast
• gypsum

Grind and add some of the powder to the jar.

Grind them up in the blender in a jar

For each jar combine:

• 14g honey
• 500g / ml of water
• Stirrer bar

Stir until honey is completely dissolved.

Pressure cook 15# pressure for 20 minutes. Steamer rack to raise the jars above the water is recommended. Another source suggested longer, but don’t overcook or else the sugars will caremelize

To inoculate:

Sterile environment: Still air box or In front of flow hood. If no sterila environment is available, best to use a syringe.

If using syringe, inoculate 1-1.5 cc

If using plate, using sterile scalpel, cut a section of the petri dish. Don’t competely open the dish or the jar. In the video, he has the jar already unscrewed so he could just lift the jar, drop the culture in, and close it.

keep at 70 degrees in low traffic environment.

Agitate daily for 1-2 weeks with the stir plate.

Test the culture